Dr Crossett began his scientific career at the University of Manchester, gaining a BSc(Hons) in Biochemistry, followed by a PhD at the University of Cambridge under the mentorship of Dr F Stewart, in which he characterised a novel uterine protein in mares. He completed post-doctoral research at Imperial College, London investigating protein based tumour therapies and subsequently utilised a proteomics-based approach to discover novel anti-microbial and vaccine targets in Burkholderia pseudomallei. In 2004, he became the Research Fellow in Proteomics and Manager of the Sydney University Proteome Research Unit.